Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells
Joint Authors
Eftekhari-Yazdi, Poopak
Dalman, Azam
Sepehri, Houri
Sadeghian-Nodoushan, Fatemeh
Imani, Husayn
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study was evaluated the effect of different synchronization protocols such as serum starvation for 1–3 days, confluency and chemical inhibitors on synchronization accuracy at G0/G1, apoptosis, and DNA synthesis in sheep granulosa cells.
The cells were obtained from ovarian antral follicles of slaughtered sheep and used at first and fifth passages.
Flow cytometry analysis showed that confluent cells, serum starvation for 24, 48, and 72 hours, and mimosine treatment significantly increased G0/G1 phase cells when compared to normally growing cells (P<0.05).
Nocodazole treatment increased the cell population in the G0/G1 stage when compared with the control group but did not change the G2/M stage population.
Treatment of cells with mimosine, nocodazole, and serum starvation in three groups resulted in proliferation arrest (P<0.05).
Serum starvation for 72 hours significantly promoted apoptosis in granulosa cells (P<0.05).
The results of the primary culture and 5th passage were the same.
The use of 48-hour serum starvation and mimosine treatments has been recommended because cell death in these groups was very similar to the control group.
American Psychological Association (APA)
Sadeghian-Nodoushan, Fatemeh& Eftekhari-Yazdi, Poopak& Dalman, Azam& Imani, Husayn& Sepehri, Houri. 2014. Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells. Chinese Journal of Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-503342
Modern Language Association (MLA)
Sadeghian-Nodoushan, Fatemeh…[et al.]. Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells. Chinese Journal of Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-503342
American Medical Association (AMA)
Sadeghian-Nodoushan, Fatemeh& Eftekhari-Yazdi, Poopak& Dalman, Azam& Imani, Husayn& Sepehri, Houri. Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells. Chinese Journal of Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-503342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503342