Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters
Joint Authors
Thelaus, J.
Bäck, E.
Mattsson, R.
Wikström, P.
Broman, T.
Forsman, Mats
Eliasson, H.
Granberg, M.
Sjöstedt, A.
Karlsson, L.
Andersson, A.-C.
Larsson, E.
Bäckman, S.
Source
International Journal of Microbiology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Tularemia, caused by the bacterium Francisella tularensis, where F.
tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden.
Despite this, our understanding of the natural life-cycle of the organism is still limited.
During three years, we collected surface water samples (n=341) and sediment samples (n=245) in two areas in Sweden with endemic tularemia.
Real-time PCR screening demonstrated the presence of F.
tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively.
The 16S rRNA sequences from those samples all grouped to the species F.
tularensis.
Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F.
tularensis subspecies holarctica-specific sequences.
These sequences were detected in water sampled during both outbreak and nonoutbreak years.
Our results indicate that diverse F.
tularensis-like organisms, including F.
tularensis subsp.
holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia.
American Psychological Association (APA)
Broman, T.& Thelaus, J.& Andersson, A.-C.& Bäckman, S.& Wikström, P.& Larsson, E.…[et al.]. 2010. Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-503364
Modern Language Association (MLA)
Broman, T.…[et al.]. Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters. International Journal of Microbiology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-503364
American Medical Association (AMA)
Broman, T.& Thelaus, J.& Andersson, A.-C.& Bäckman, S.& Wikström, P.& Larsson, E.…[et al.]. Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters. International Journal of Microbiology. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-503364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503364
