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Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp2623)
Joint Authors
Alunda, José María
Valladares, Basilio
Martínez, Enrique
García-Coiradas, Leticia
Cuquerella, Montserrat
de la Fuente, Concepción
Angulo-Cubillán, Francisco
Source
Veterinary Medicine International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Haemonchosis, caused by the abomasal nematode Haemonchus contortus, is a common parasitic disease of sheep.
Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge.
Recombinant DNA technology was applied to obtain a synthetic protein (rHcp26/23).
Immunological assays (ELISA, Western blotting, and immunolocalization), using sera from lambs immunized with p26/23, confirmed the identity of the recombinant protein and demonstrated that the synthetic protein is equivalent to the purified protein employed in the previous immunoprophylaxis studies.
Vaccination of lambs with 300 μg of rHcp26/23 and Freund's adjuvant elicited a notable specific antibody response.
Immunization did not induce any significant protection after challenge with 16000 infective larvae of H.
contortus, and comparable values for parasite faecal egg output, packed cell volume, and abomasal parasite burdens were found in vaccinated and control animals.
American Psychological Association (APA)
García-Coiradas, Leticia& Angulo-Cubillán, Francisco& Valladares, Basilio& Martínez, Enrique& de la Fuente, Concepción& Alunda, José María…[et al.]. 2010. Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp2623). Veterinary Medicine International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-503388
Modern Language Association (MLA)
García-Coiradas, Leticia…[et al.]. Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp2623). Veterinary Medicine International No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-503388
American Medical Association (AMA)
García-Coiradas, Leticia& Angulo-Cubillán, Francisco& Valladares, Basilio& Martínez, Enrique& de la Fuente, Concepción& Alunda, José María…[et al.]. Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp2623). Veterinary Medicine International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-503388
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503388