Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor
Joint Authors
Hatzoglou, C.
Kouritas, V. K.
Evaggelopoulos, K.
Gourgoulianis, K. I.
Foroulis, C. N.
Molyvdas, P.-A.
Ioannou, Maria
Desimonas, N.
Source
Experimental Diabetes Research
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Insulin directly changes the sheep pleural electrophysiology.
The aim of this study was to investigate whether insulin induces similar effects in human pleura, to clarify insulin receptor's involvement, and to demonstrate if glibenclamide (hypoglycemic agent) reverses this effect.
Methods.
Human parietal pleural specimens were mounted in Ussing chambers.
Solutions containing insulin or glibenclamide and insulin with anti-insulin antibody, anti-insulin receptor antibody, and glibenclamide were used.
The transmesothelial resistance (RTM) was determined.
Immunohistochemistry for the presence of Insulin Receptors (IRa, IRb) was also performed.
Results.
Insulin increased RTM within 1st min (P=.016), when added mesothelially which was inhibited by the anti-insulin and anti-insulin receptor antibodies.
Glibenclamide also eliminated the insulin-induced changes.
Immunohistochemistry verified the presence of IRa and IRb.
Conclusion.
Insulin induces electrochemical changes in humans as in sheep via interaction with its receptor.
This effect is abolished by glibenclamide.
American Psychological Association (APA)
Kouritas, V. K.& Ioannou, Maria& Foroulis, C. N.& Desimonas, N.& Evaggelopoulos, K.& Gourgoulianis, K. I.…[et al.]. 2010. Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor. Experimental Diabetes Research،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-503486
Modern Language Association (MLA)
Kouritas, V. K.…[et al.]. Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor. Experimental Diabetes Research No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-503486
American Medical Association (AMA)
Kouritas, V. K.& Ioannou, Maria& Foroulis, C. N.& Desimonas, N.& Evaggelopoulos, K.& Gourgoulianis, K. I.…[et al.]. Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor. Experimental Diabetes Research. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-503486
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503486