![](/images/graphics-bg.png)
A Novel Tetrapeptide Derivative Exhibits In Vitro Inhibition of Neutrophil-Derived Reactive Oxygen Species and Lysosomal Enzymes Release
Joint Authors
Ramanujam, Meera
Miriyala, Sumitra
Puvanakrishnan, Rengarajulu
Panchatcharam, Manikandan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Neutrophil infiltration plays a major role in the pathogenesis of myocardial injury.
Oxidative injury is suggested to be a central mechanism of the cellular damage after acute myocardial infarction.
This study is pertained to the prognostic role of a tetrapeptide derivative PEP1261 (BOC-Lys(BOC)-Arg-Asp-Ser(tBu)-OtBU), a peptide sequence (39–42) of lactoferrin, studied in the modulation of neutrophil functions in vitro by measuring the reactive oxygen species (ROS) generation, lysosomal enzymes release, and enhanced expression of C proteins.
The groundwork experimentation was concerned with the isolation of neutrophils from the normal and acute myocardial infarct rats to find out the efficacy of PEP1261 in the presence of a powerful neutrophil stimulant, phorbol 12-myristate 13 acetate (PMA).
Stimulation of neutrophils with PMA resulted in an oxidative burst of superoxide anion and enhanced release of lysosomal enzymes and expression of complement proteins.
The present study further demonstrated that the free radicals increase the complement factors in the neutrophils confirming the role of ROS.
PEP1261 treatment significantly reduced the levels of superoxide anion and inhibited the release of lysosomal enzymes in the stimulated control and infarct rat neutrophils.
This study demonstrated that PEP1261 significantly inhibited the effect on the ROS generation as well as the mRNA synthesis and expression of the complement factors in neutrophils isolated from infarct heart.
American Psychological Association (APA)
Miriyala, Sumitra& Panchatcharam, Manikandan& Ramanujam, Meera& Puvanakrishnan, Rengarajulu. 2013. A Novel Tetrapeptide Derivative Exhibits In Vitro Inhibition of Neutrophil-Derived Reactive Oxygen Species and Lysosomal Enzymes Release. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503488
Modern Language Association (MLA)
Miriyala, Sumitra…[et al.]. A Novel Tetrapeptide Derivative Exhibits In Vitro Inhibition of Neutrophil-Derived Reactive Oxygen Species and Lysosomal Enzymes Release. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-503488
American Medical Association (AMA)
Miriyala, Sumitra& Panchatcharam, Manikandan& Ramanujam, Meera& Puvanakrishnan, Rengarajulu. A Novel Tetrapeptide Derivative Exhibits In Vitro Inhibition of Neutrophil-Derived Reactive Oxygen Species and Lysosomal Enzymes Release. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503488