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Acute In Vivo Response to an Alternative Implant for Urogynecology
Joint Authors
MacNeil, Sheila
Chapple, Christopher R.
Manodoro, Stefano
Albersen, Maarten
Deprest, Jan
Roman Regueros, Sabiniano
Osman, Nadir I.
Bullock, Anthony J.
Zia, Silvia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
To investigate in vivo the acute host response to an alternative implant designed for the treatment of stress urinary incontinence (SUI) and pelvic organ prolapse (POP).
Methods.
A biodegradable scaffold was produced from poly-L-lactic acid (PLA) using the electrospinning technique.
Human and rat adipose-derived stem cells (ADSCs) were isolated and characterized by fluorescence-activated cell sorting and differentiation assays.
PLA scaffolds were seeded and cultured for 2 weeks with human or rat ADSCs.
Scaffolds with and without human or rat ADSCs were implanted subcutaneously on the abdominal wall of rats.
After 3 and 7 days, 6 animals from each group were sacrificed.
Sections from each sample were analyzed by Haematoxylin and Eosin staining, Sirius red staining, and immunohistochemistry for CD68, PECAM-1, and collagen I and III.
Results.
Animals responded to the scaffolds with an acute macrophage response.
After 7 days of implantation, there was extensive host cell penetration, new blood vessel formation, and new collagen deposition throughout the full thickness of the samples without obvious differences between cell-containing and cell-free scaffolds.
Conclusions.
The acute in vivo response to an alternative implant (both with and without cells) for the treatment of SUI and POP showed good acute integration into the host tissues.
American Psychological Association (APA)
Roman Regueros, Sabiniano& Albersen, Maarten& Manodoro, Stefano& Zia, Silvia& Osman, Nadir I.& Bullock, Anthony J.…[et al.]. 2014. Acute In Vivo Response to an Alternative Implant for Urogynecology. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503524
Modern Language Association (MLA)
Roman Regueros, Sabiniano…[et al.]. Acute In Vivo Response to an Alternative Implant for Urogynecology. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-503524
American Medical Association (AMA)
Roman Regueros, Sabiniano& Albersen, Maarten& Manodoro, Stefano& Zia, Silvia& Osman, Nadir I.& Bullock, Anthony J.…[et al.]. Acute In Vivo Response to an Alternative Implant for Urogynecology. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503524
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503524