Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Joint Authors
Newcomer, Robert G.
Keller, Thomas C. S.
Schlenoff, Joseph B.
Sang, Qing-Xiang Amy
Moussallem, Maroun D.
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-29
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Information Technology and Computer Science
Abstract EN
Under normal physiological conditions, mature human coronary artery smooth muscle cells (hCASMCs) exhibit a “contractile” phenotype marked by low rates of proliferation and protein synthesis, but these cells possess the remarkable ability to dedifferentiate into a “synthetic” phenotype when stimulated by conditions of pathologic stress.
A variety of polyelectrolyte multilayer (PEMU) films are shown here to exhibit bioactive properties that induce distinct responses from cultured hCASMCs.
Surfaces terminated with Nafion or poly(styrenesulfonic acid) (PSS) induce changes in the expression and organization of intracellular proteins, while a hydrophilic, zwitterionic copolymer of acrylic acid and 3-[2-(acrylamido)-ethyl dimethylammonio] propane sulfonate (PAA-co-PAEDAPS) is resistant to cell attachment and suppresses the formation of key cytoskeletal components.
Differential expression of heat shock protein 90 and actin is observed, in terms of both their magnitude and cellular localization, and distinct cytoplasmic patterns of vimentin are seen.
The ionophore A23187 induces contraction in confluent hCASMC cultures on Nafion-terminated surfaces.
These results demonstrate that PEMU coatings exert direct effects on the cytoskeletal organization of attaching hCASMCs, impeding growth in some cases, inducing changes consistent with phenotypic modulation in others, and suggesting potential utility for PEMU surfaces as a coating for coronary artery stents and other implantable medical devices.
American Psychological Association (APA)
Newcomer, Robert G.& Moussallem, Maroun D.& Keller, Thomas C. S.& Schlenoff, Joseph B.& Sang, Qing-Xiang Amy. 2010. Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-503578
Modern Language Association (MLA)
Newcomer, Robert G.…[et al.]. Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces. Biotechnology Research International No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-503578
American Medical Association (AMA)
Newcomer, Robert G.& Moussallem, Maroun D.& Keller, Thomas C. S.& Schlenoff, Joseph B.& Sang, Qing-Xiang Amy. Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces. Biotechnology Research International. 2010. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-503578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503578