Evaluation of Melanogenesis in A-375 Cells in the Presence of DMSO and Analysis of Pyrolytic Profile of Isolated Melanin
Joint Authors
Kurkiewicz, Sławomir
Orchel, Joanna
Dzierżewicz, Zofia
Orchel, Arkadiusz
Chodurek, Ewa
Gawlik, Natalia
Stępień, Krystyna
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The increase of a skin malignant melanoma (melanoma malignum) incidence in the world has been observed in recent years.
The tumour, especially in advanced stadium with metastases, is highly resistant to conventional treatment.
One of the strategies is to modulate melanogenesis using chemical compounds.
In this study, the processes of differentiation and melanogenesis induced by dimethylsulfoxide (DMSO) in human melanoma cells (A-375) were investigated.
Natural melanin isolated from A-375 melanoma cell line treated with 0.3% DMSO was analyzed by pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) method.
The products derived from pheomelanin have not been stated in the pyrolytic profile of analyzed melanin.
Within all products derived from eumelanins, 1,2-benzenediol has been predominated.
It has been shown that in the melanoma cells stimulated with 0.3% and 1% DMSO, the increase of transcriptional activity of the tyrosinase gene took place.
It was accompanied by the rise of tyrosinase activity and an accumulation of melanin in the cells.
The better knowledge about the structure of melanins can contribute to establish the uniform criteria of malignant melanoma morbidity risk.
American Psychological Association (APA)
Chodurek, Ewa& Orchel, Arkadiusz& Orchel, Joanna& Kurkiewicz, Sławomir& Gawlik, Natalia& Dzierżewicz, Zofia…[et al.]. 2012. Evaluation of Melanogenesis in A-375 Cells in the Presence of DMSO and Analysis of Pyrolytic Profile of Isolated Melanin. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-503581
Modern Language Association (MLA)
Chodurek, Ewa…[et al.]. Evaluation of Melanogenesis in A-375 Cells in the Presence of DMSO and Analysis of Pyrolytic Profile of Isolated Melanin. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-503581
American Medical Association (AMA)
Chodurek, Ewa& Orchel, Arkadiusz& Orchel, Joanna& Kurkiewicz, Sławomir& Gawlik, Natalia& Dzierżewicz, Zofia…[et al.]. Evaluation of Melanogenesis in A-375 Cells in the Presence of DMSO and Analysis of Pyrolytic Profile of Isolated Melanin. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-503581
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503581