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High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct
Joint Authors
Leite, Selma Gomes Ferreira
de Castro, Aline Machado
Pereira, Nei
Carvalho, Daniele Fernandes
Pedro, Kelly Cristina Nascimento Rodrigues
da Cruz, Juliana Cunha
Ferreira, Marcela Costa
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol.
In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation.
This fungal strain produced high contents of endoglucanase activity (6,358 U·L−1) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L−1, resp.).
The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50∘C, accessory xylanase activity, and absence of proteolytic activity towards azocasein.
This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.
American Psychological Association (APA)
de Castro, Aline Machado& Ferreira, Marcela Costa& da Cruz, Juliana Cunha& Pedro, Kelly Cristina Nascimento Rodrigues& Carvalho, Daniele Fernandes& Leite, Selma Gomes Ferreira…[et al.]. 2010. High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct. Enzyme Research،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-503615
Modern Language Association (MLA)
de Castro, Aline Machado…[et al.]. High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct. Enzyme Research No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-503615
American Medical Association (AMA)
de Castro, Aline Machado& Ferreira, Marcela Costa& da Cruz, Juliana Cunha& Pedro, Kelly Cristina Nascimento Rodrigues& Carvalho, Daniele Fernandes& Leite, Selma Gomes Ferreira…[et al.]. High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-503615
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503615