Rapid Enzymatic Method for Pectin Methyl Esters Determination
Joint Authors
Georgakopoulos, Dimitrios G.
Vasiliou, Efstathios G.
Yialouris, Constantinos P.
Georgiou, Constantinos A.
Łękawska-Andrinopoulou, Lucyna
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Pectin is a natural polysaccharide used in food and pharma industries.
Pectin degree of methylation is an important parameter having significant influence on pectin applications.
A rapid, fully automated, kinetic flow method for determination of pectin methyl esters has been developed.
The method is based on a lab-made analyzer using the reverse flow-injection/stopped flow principle.
Methanol is released from pectin by pectin methylesterase in the first mixing coil.
Enzyme working solution is injected further downstream and it is mixed with pectin/pectin methylesterase stream in the second mixing coil.
Methanol is oxidized by alcohol oxidase releasing formaldehyde and hydrogen peroxide.
This reaction is coupled to horse radish peroxidase catalyzed reaction, which gives the colored product 4-N-(p-benzoquinoneimine)-antipyrine.
Reaction rate is proportional to methanol concentration and it is followed using Ocean Optics USB 2000+ spectrophotometer.
The analyzer is fully regulated by a lab written LabVIEW program.
The detection limit was 1.47 mM with an analysis rate of 7 samples h−1.
A paired t-test with results from manual method showed that the automated method results are equivalent to the manual method at the 95% confidence interval.
The developed method is rapid and sustainable and it is the first application of flow analysis in pectin analysis.
American Psychological Association (APA)
Łękawska-Andrinopoulou, Lucyna& Vasiliou, Efstathios G.& Georgakopoulos, Dimitrios G.& Yialouris, Constantinos P.& Georgiou, Constantinos A.. 2013. Rapid Enzymatic Method for Pectin Methyl Esters Determination. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503650
Modern Language Association (MLA)
Łękawska-Andrinopoulou, Lucyna…[et al.]. Rapid Enzymatic Method for Pectin Methyl Esters Determination. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-503650
American Medical Association (AMA)
Łękawska-Andrinopoulou, Lucyna& Vasiliou, Efstathios G.& Georgakopoulos, Dimitrios G.& Yialouris, Constantinos P.& Georgiou, Constantinos A.. Rapid Enzymatic Method for Pectin Methyl Esters Determination. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503650
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503650