Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin
Joint Authors
Igarashi, Naoko
Toida, Toshihiko
Sakai, Shinobu
Takeguchi, Atsuko
Akiyama, Hiroshi
Higashi, Kyohei
Source
International Journal of Carbohydrate Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chondroitin sulfate (CS) is a glycosaminoglycan (GAG) side chain of proteoglycans (PGs) which are widely distributed in the extracellular matrix and at cell surface.
CS shows a highly structural diversity in not only molecular weight (MW) but sulfonation pattern.
CS has been reported to exert anti-inflammatory activity by having effects on cytokine production by helper T cells.
In this study, we focused on the structures of CS chains, especially MW of CS, and investigated effect of the different MW of CS on binding affinity with L-selectin and cytokine production by murine splenocytes.
Firstly, we fractionated CS by employing gel filtration chromatography and obtained several CS fractions with different MW.
Then the interaction between fractionated CS and L-selectin was analyzed by surface plasmon resonance (SPR).
Finally, the influence of MW of CS on cytokine production by murine splenocytes was investigated in vitro.
The results showed that interferon-gamma production was significantly increased by mouse splenocytes cocultivated with CS.
On the contrary, CS inhibited interleukin 5 production by murine splenocytes depending on MW of the cocultivated CS.
These results strongly indicate the existence of the optimal molecular size for an anti-inflammatory effect of CS through cytokine production by murine splenocytes.
American Psychological Association (APA)
Igarashi, Naoko& Takeguchi, Atsuko& Sakai, Shinobu& Akiyama, Hiroshi& Higashi, Kyohei& Toida, Toshihiko. 2013. Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin. International Journal of Carbohydrate Chemistry،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-503705
Modern Language Association (MLA)
Igarashi, Naoko…[et al.]. Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin. International Journal of Carbohydrate Chemistry No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-503705
American Medical Association (AMA)
Igarashi, Naoko& Takeguchi, Atsuko& Sakai, Shinobu& Akiyama, Hiroshi& Higashi, Kyohei& Toida, Toshihiko. Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin. International Journal of Carbohydrate Chemistry. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-503705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503705