A New ELISA for Dermatomyositis Autoantibodies : Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement
Joint Authors
Muro, Yoshinao
Akiyama, Masashi
Sugiura, Kazumitsu
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Advances in immunology, biochemistry, and molecular biology have enabled the development of a number of assays for measuring autoantibodies.
ELISA has been widely used, because it can deal with relatively large numbers of serum samples more quickly than other immunologic methods, such as immunoblotting and immunoprecipitation.
Recombinant autoantigens, which are generally produced in E.
coli using the relevant cloned cDNA, are necessary for ELISA.
Conventional clinical ELISA tests are limited in their ability to purify proteins free of bacterial contaminants, and the process is labor intensive.
We recently developed new ELISA tests that utilize simple in vitro transcription and translation labeling of autoantigens in order to measure dermatomyositis- (DM-) specific autoantibodies, including autoantibodies to Mi-2, MDA5, NXP-2, TIF1-α, and TIF1-γ.
This method may allow for the rapid conversion of cDNAs to a chemiluminescent ELISA to detect autoantibodies that are found not only in DM but also in other autoimmune diseases.
American Psychological Association (APA)
Muro, Yoshinao& Sugiura, Kazumitsu& Akiyama, Masashi. 2013. A New ELISA for Dermatomyositis Autoantibodies : Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503757
Modern Language Association (MLA)
Muro, Yoshinao…[et al.]. A New ELISA for Dermatomyositis Autoantibodies : Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement. Clinical and Developmental Immunology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-503757
American Medical Association (AMA)
Muro, Yoshinao& Sugiura, Kazumitsu& Akiyama, Masashi. A New ELISA for Dermatomyositis Autoantibodies : Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-503757
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503757