The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site
Joint Authors
Sugimoto, Naoki
Miyoshi, Daisuke
Fujimoto, Takeshi
Nakano, Shu-ichi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Both cellular environmental factors and chemical modifications critically affect the properties of nucleic acids.
However, the structure and stability of DNA containing abasic sites under cell-mimicking molecular crowding conditions remain unclear.
Here, we investigated the molecular crowding effects on the structure and stability of the G-quadruplexes including a single abasic site.
Structural analysis by circular dichroism showed that molecular crowding by PEG200 did not affect the topology of the G-quadruplex structure with or without an abasic site.
Thermodynamic analysis further demonstrated that the degree of stabilization of the G-quadruplex by molecular crowding decreased with substitution of an abasic site for a single guanine.
Notably, we found that the molecular crowding effects on the enthalpy change for G-quadruplex formation had a linear relationship with the abasic site effects depending on its position.
These results are useful for predicting the structure and stability of G-quadruplexes with abasic sites in the cell-mimicking conditions.
American Psychological Association (APA)
Fujimoto, Takeshi& Nakano, Shu-ichi& Miyoshi, Daisuke& Sugimoto, Naoki. 2011. The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-503794
Modern Language Association (MLA)
Fujimoto, Takeshi…[et al.]. The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site. Journal of Nucleic Acids No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-503794
American Medical Association (AMA)
Fujimoto, Takeshi& Nakano, Shu-ichi& Miyoshi, Daisuke& Sugimoto, Naoki. The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-503794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503794