BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules : Molecular Cloning, Preparation, and In Vitro Analysis
Joint Authors
Shum-Tim, Dominique
Prakash, Satya
Khan, Afshan Afsar
Paul, Arghya
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The potential of genetically modified cardiomyoblasts in treating damaged myocardium is well known.
However, efficient delivery of these cells is of major concern during treatment.
The limiting factors are the massive cell death that occurs soon after their intramyocardial transplantation into the beating heart.
To address these problems, we generated recombinant baculoviruses (BacMam viruses) which efficiently transduced cardiomyoblast cells under optimized conditions.
These genetically modified cells were then protected in a new polymeric microcapsule using poly-ethylene-glycol (PEG), alginate, and poly-L-lysine (PLL) polymers for efficient delivery.
Results showed that microcapsules maintain cell viability and support cell proliferation for at least 30 days.
The capsules exhibit strong immunoprotective potential and have high mechanical and osmotic stability with more than 70% intact capsules.
The encased transduced cells showed a rapid transgene expression inside the capsule for at least 15 days.
However, preclinical studies are needed to further explore its long-term functional benefits.
American Psychological Association (APA)
Paul, Arghya& Khan, Afshan Afsar& Shum-Tim, Dominique& Prakash, Satya. 2011. BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules : Molecular Cloning, Preparation, and In Vitro Analysis. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-503885
Modern Language Association (MLA)
Paul, Arghya…[et al.]. BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules : Molecular Cloning, Preparation, and In Vitro Analysis. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-503885
American Medical Association (AMA)
Paul, Arghya& Khan, Afshan Afsar& Shum-Tim, Dominique& Prakash, Satya. BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules : Molecular Cloning, Preparation, and In Vitro Analysis. Journal of Biomedicine and Biotechnology. 2011. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-503885
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503885