Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-κB Activity, and Its Related Cytokines in Rats with Open Abdominal Injury
Joint Authors
Zhou, Song
Zhang, Xing Jian
Wang, Ya Li
Xue, Xiaojun
Zheng, Jun
Liu, Qiang
Zhang, Wen Hua
Source
Gastroenterology Research and Practice
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To investigate the role of oxidative stress, NF-κB activity, and its related cytokines in the pathogenesis of seawater immersion after open abdominal injury (SI-OAI) and whether UTI treatment can attenuate SI-OAI induced IMI.
Methods.
Wistar rats were randomly divided into three groups: C group, S group, and U group.
The rats in C group only suffered from anesthesia and surgical operation, whereas the rats in S group and U group received caudal vein injection of normal saline without/with 50,000 U/kg body weight of UTI.
The activities of TNF-α, IL-6, SOD, MDA, ROS, NF-κB, and IκB-β were monitored by ELISA, biochemical methods, EMSA, and Western blot, respectively.
Results.
The plasma inflammatory mediators and the contents of MDA, ROS, and NF-κB in intestine as well as the pathological scores in ileal mucosa were significantly increased in rats after SI-OAI, accompanied by a reduction in SOD activities and IκB-β levels.
UTI treatment significantly attenuated intestinal histopathological changes with evidence of a decrease in all of the parameters, except for upregulation of the levels of SOD and IκB-β protein.
Conclusion.
UTI can attenuate SI-OAI induced IMI via inhibition of NF-κB activity, subsequently inhibiting the expression of inflammatory cytokines and by combating oxidative stress.
American Psychological Association (APA)
Zhang, Xing Jian& Wang, Ya Li& Zhou, Song& Xue, Xiaojun& Liu, Qiang& Zhang, Wen Hua…[et al.]. 2014. Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-κB Activity, and Its Related Cytokines in Rats with Open Abdominal Injury. Gastroenterology Research and Practice،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503899
Modern Language Association (MLA)
Zhang, Xing Jian…[et al.]. Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-κB Activity, and Its Related Cytokines in Rats with Open Abdominal Injury. Gastroenterology Research and Practice No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-503899
American Medical Association (AMA)
Zhang, Xing Jian& Wang, Ya Li& Zhou, Song& Xue, Xiaojun& Liu, Qiang& Zhang, Wen Hua…[et al.]. Urinary Trypsin Inhibitor Ameliorates Seawater Immersion-Induced Intestinal Mucosa Injury via Antioxidation, Modulation of NF-κB Activity, and Its Related Cytokines in Rats with Open Abdominal Injury. Gastroenterology Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503899
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503899