Cellulolytic Enzymes Production via Solid-State Fermentation : Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate
Joint Authors
Vadlani, Praveen V.
Brijwani, Khushal
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We investigated the effect of pretreatment on the physicochemical characteristics—crystallinity, bed porosity, and volumetric specific surface of soybean hulls and production of cellulolytic enzymes in solid-state fermentation of Trichoderma reesei and Aspergillus oryzae cultures.
Mild acid and alkali and steam pretreatments significantly increased crystallinity and bed porosity without significant change inholocellulosic composition of substrate.
Crystalline and porous steam-pretreated soybean hulls inoculated with T.
reesei culture had 4 filter paper units (FPU)/g-ds, 0.6 IU/g-ds β-glucosidase, and 45 IU/g-ds endocellulase, whereas untreated hulls had 0.75 FPU/g-ds, 0.06 IU/g-ds β-glucosidase, and 7.29 IU/g-ds endocellulase enzyme activities.
In A.
oryzae steam-pretreated soybean hulls had 47.10 IU/g-ds endocellulase compared to 30.82 IU/g-ds in untreated soybean hulls.
Generalized linear statistical model fitted to enzyme activity data showed that effects of physicochemical characteristics on enzymes production were both culture and enzyme specific.
The paper shows a correlation between substrate physicochemical properties and enzyme production.
American Psychological Association (APA)
Brijwani, Khushal& Vadlani, Praveen V.. 2011. Cellulolytic Enzymes Production via Solid-State Fermentation : Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-504048
Modern Language Association (MLA)
Brijwani, Khushal& Vadlani, Praveen V.. Cellulolytic Enzymes Production via Solid-State Fermentation : Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-504048
American Medical Association (AMA)
Brijwani, Khushal& Vadlani, Praveen V.. Cellulolytic Enzymes Production via Solid-State Fermentation : Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-504048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504048