Increased Small Dense LDL and Decreased Paraoxonase Enzyme Activity Reveals Formation of an Atherogenic Risk in Streptozotocin-Induced Diabetic Guinea Pigs

Joint Authors

Ozcan, Filiz
Kucuksayan, Ertan
Aslan, Mutay

Source

Journal of Diabetes Research

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-03-27

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Diseases
Medicine

Abstract EN

This study aimed to investigate LDL subfraction distribution as well as serum cholesteryl ester transfer protein (CETP), lecithin-cholesterol acyltransferase (LCAT), and paraoxonase (PON1) activity in streptozotocin-induced diabetic guinea pigs.

Materials/Methods.

Guinea pigs were given a single intraperitoneal (ip) injection of streptozotocin (STZ) and animals having fasting blood glucose levels greater than 200 mg/dl, were considered diabetic.

Protein levels of LCAT and CETP were determined via ELISA.

Paraoxonase activity was measured kinetically by the formation of phenol while LDL subfraction analysis was done by disc polyacrylamide gel electrophoresis.

Results.

Plasma glucose and high-density lipoprotein (HDL) cholesterol were significantly increased while total cholesterol and LDL cholesterol were significantly decreased in diabetic guinea pigs compared to controls.

LDL subfraction analysis revealed a significant decrease in nonatherogenic LDL-2 subfraction and a significant increase in atherogenic LDL-4 subfraction in diabetic guinea pigs compared to controls.

Plasma CETP and PON1 levels were significantly decreased while LCAT showed no significant difference in diabetic guinea pigs compared to controls.

Conclusion.

Decreased non-atherogenic LDL-1, LDL-2 subfractions, increased small dense LDL-4 subfraction, and decreased PON1 activity, reveals formation of an atherogenic risk in diabetic guinea pigs.

Decrease in CETP levels supports the observed increase in HDL cholesterol levels in diabetic guinea pigs.

American Psychological Association (APA)

Aslan, Mutay& Ozcan, Filiz& Kucuksayan, Ertan. 2013. Increased Small Dense LDL and Decreased Paraoxonase Enzyme Activity Reveals Formation of an Atherogenic Risk in Streptozotocin-Induced Diabetic Guinea Pigs. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-504056

Modern Language Association (MLA)

Aslan, Mutay…[et al.]. Increased Small Dense LDL and Decreased Paraoxonase Enzyme Activity Reveals Formation of an Atherogenic Risk in Streptozotocin-Induced Diabetic Guinea Pigs. Journal of Diabetes Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-504056

American Medical Association (AMA)

Aslan, Mutay& Ozcan, Filiz& Kucuksayan, Ertan. Increased Small Dense LDL and Decreased Paraoxonase Enzyme Activity Reveals Formation of an Atherogenic Risk in Streptozotocin-Induced Diabetic Guinea Pigs. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-504056

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-504056