Multipyrene Tandem Probes for Point Mutations Detection in DNA
Joint Authors
Meschaninova, Mariya I.
Venyaminova, Alya G.
Novopashina, Darya S.
Kholodar, Svetlana A.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Here we report design, synthesis and characterization of highly sensitive, specific and stable in biological systems fluorescent probes for point mutation detection in DNA.
The tandems of 3′- and 5′-mono- and bis-pyrene conjugated oligo(2′-O-methylribonucleotides), protected by 3′-“inverted” thymidine, were constructed and their potential as new instruments for genetic diagnostics was studied.
Novel probes have been shown to exhibit an ability to form stable duplexes with DNA target due to the stabilizing effect of multiple pyrene units at the junction.
The relationship between fluorescent properties of developed probes, the number of pyrene residues at the tandem junction, and the location of point mutation has been studied.
On the basis of the data obtained, we have chosen the probes possessing the highest fluorescence intensity along with the best mismatch discrimination and deletion and insertion detection ability.
Application of developed probes for detection of polymorphism C677T in MTHFR gene has been demonstrated on model systems.
American Psychological Association (APA)
Kholodar, Svetlana A.& Novopashina, Darya S.& Meschaninova, Mariya I.& Venyaminova, Alya G.. 2013. Multipyrene Tandem Probes for Point Mutations Detection in DNA. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-504084
Modern Language Association (MLA)
Kholodar, Svetlana A.…[et al.]. Multipyrene Tandem Probes for Point Mutations Detection in DNA. Journal of Nucleic Acids No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-504084
American Medical Association (AMA)
Kholodar, Svetlana A.& Novopashina, Darya S.& Meschaninova, Mariya I.& Venyaminova, Alya G.. Multipyrene Tandem Probes for Point Mutations Detection in DNA. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-504084
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504084