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Chemokine Receptor and Ligand Upregulation in the Diaphragm during Endotoxemia and Pseudomonas Lung Infection
Joint Authors
Demoule, Alexandre
Petrof, Basil J.
Yahiaoui, Linda
Danialou, Gawiyou
Divangahi, Maziar
Gvozdic, Dusanka
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-04-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Sepsis-induced diaphragmatic inflammation has been associated with respiratory failure, but the role of chemokines in this process has not been evaluated.
Here we sought to study the local expression and molecular regulation of the chemokines, regulated upon activation normal T cell expressed and secreted (RANTES) and macrophage inflammatory protein (MIP)-1α, in the murine diaphragm during sepsis.
Constitutive expression levels of RANTES and MIP-1α, as well as their receptors, CCR1 and CCR5, were significantly higher in diaphragm than limb muscle.
Sepsis was induced by acute lipopolysaccharide (LPS) delivery or subacutely by intratracheal administration of live Pseudomonas aeruginosa bacteria.
Both sepsis models triggered a marked upregulation of RANTES and MIP-1α in the diaphragm.
In vitro, stimulation of diaphragmatic muscle cells with LPS also led to RANTES upregulation.
Inhibition of the NF-kB pathway using pharmacologic or dominant negative genetic approaches blocked the LPS-induced RANTES upregulation, while free radical scavengers had no effect.
We conclude that sepsis leads to greatly increased expression of RANTES, MIP-1α and their cognate receptors in the diaphragm.
Manipulation of the NF-kB pathway and other regulators of chemokine expression in the diaphragm could represent a novel method for mitigating the skeletal muscle inflammatory response associated with sepsis-induced diaphragmatic dysfunction.
American Psychological Association (APA)
Demoule, Alexandre& Divangahi, Maziar& Yahiaoui, Linda& Danialou, Gawiyou& Gvozdic, Dusanka& Petrof, Basil J.. 2009. Chemokine Receptor and Ligand Upregulation in the Diaphragm during Endotoxemia and Pseudomonas Lung Infection. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-504100
Modern Language Association (MLA)
Demoule, Alexandre…[et al.]. Chemokine Receptor and Ligand Upregulation in the Diaphragm during Endotoxemia and Pseudomonas Lung Infection. Mediators of Inflammation No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-504100
American Medical Association (AMA)
Demoule, Alexandre& Divangahi, Maziar& Yahiaoui, Linda& Danialou, Gawiyou& Gvozdic, Dusanka& Petrof, Basil J.. Chemokine Receptor and Ligand Upregulation in the Diaphragm during Endotoxemia and Pseudomonas Lung Infection. Mediators of Inflammation. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-504100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504100