Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents
Joint Authors
Lee, Shi Woo
Shin, Ji Won
Shin, Jung-Woog
Jang, Ji-Yeon
Kim, Dong Hwa
Kim, Su-Hyang
Park, So Hee
Mun, ChiWoong
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects.
Recently, the roles of biomimetic microphysical environments have drawn much attention in this field.
We studied MSC differentiation and changes in gene expression in relation to osteoblast-like cell and smooth muscle-like cell type resulting from various microphysical environments, including differing magnitudes of tensile strain and substrate geometries for 8 days.
In addition, we also investigated the residual effects of those selected microphysical environment factors on the differentiation by ceasing those factors for 3 days.
The results of this study showed the effects of the strain magnitudes and surface geometries.
However, the genes which are related to the same cell type showed different responses depending on the changes in strain magnitude and surface geometry.
Also, different responses were observed three days after the straining was stopped.
These data confirm that controlling microenvironments so that they mimic those in vivo contributes to the differentiation of MSCs into specific cell types.
And duration of straining engagement was also found to play important roles along with surface geometry.
American Psychological Association (APA)
Jang, Ji-Yeon& Lee, Shi Woo& Park, So Hee& Shin, Ji Won& Mun, ChiWoong& Kim, Su-Hyang…[et al.]. 2011. Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-504111
Modern Language Association (MLA)
Jang, Ji-Yeon…[et al.]. Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-504111
American Medical Association (AMA)
Jang, Ji-Yeon& Lee, Shi Woo& Park, So Hee& Shin, Ji Won& Mun, ChiWoong& Kim, Su-Hyang…[et al.]. Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-504111
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504111