Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs
Joint Authors
Jordan, Erin T.
Lu, Yangqing
Sonnet, Corinne
Peroni, John F.
West, Jennifer L.
Davis, Alan R.
Stice, Steven L.
Olabisi, Ronke M.
Olmsted-Davis, Elizabeth A.
West, Franklin
Mumaw, Jennifer
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics.
Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation.
However, methods to make these treatments available for clinical applications are presently lacking.
In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities.
MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion.
We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation.
Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy.
These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs.
American Psychological Association (APA)
Mumaw, Jennifer& Jordan, Erin T.& Sonnet, Corinne& Olabisi, Ronke M.& Olmsted-Davis, Elizabeth A.& Davis, Alan R.…[et al.]. 2012. Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-504217
Modern Language Association (MLA)
Mumaw, Jennifer…[et al.]. Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs. International Journal of Biomaterials No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-504217
American Medical Association (AMA)
Mumaw, Jennifer& Jordan, Erin T.& Sonnet, Corinne& Olabisi, Ronke M.& Olmsted-Davis, Elizabeth A.& Davis, Alan R.…[et al.]. Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-504217
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504217