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Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
Joint Authors
Siangproh, Weena
Chantarateepra, Pimkwan
Motomizu, Shoji
Chailapakul, Orawon
Source
International Journal of Electrochemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed.
A homemade microcolumn SPE system coupled with sequential injection analysis (SIA) was used to automate the sample cleanup and extraction of sulfonamides.
The optimal flow rate of sample loading and elution was found to be 10 μL/s, and optimal elution time of zone was 20–24 s.
Under the optimal conditions, a linear relationship between peak area and sulfonamide concentrations was obtained in the range of 0.01–8.0 μg mL−1.
Detection limits for seven sulfonamides were between 1.2 ng mL−1 and 11.2 ng mL−1.
The proposed method has been applied for the determination of sulfonamides in shrimp.
Recoveries in the range of 84–107% and relative standard deviations (RSDs) below 6.5% for intraday and 13% for inter-day were received for three concentration levels of spiking.
The results showed that the present method was simple, rapid, accurate and highly sensitive for the determination of sulfonamides.
American Psychological Association (APA)
Chantarateepra, Pimkwan& Siangproh, Weena& Motomizu, Shoji& Chailapakul, Orawon. 2011. Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp. International Journal of Electrochemistry،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-504319
Modern Language Association (MLA)
Chantarateepra, Pimkwan…[et al.]. Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp. International Journal of Electrochemistry No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-504319
American Medical Association (AMA)
Chantarateepra, Pimkwan& Siangproh, Weena& Motomizu, Shoji& Chailapakul, Orawon. Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp. International Journal of Electrochemistry. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-504319
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504319