Selective Gene Expression Analysis of Muscular and Vascular Components in Hearts Using Laser Microdissection Method
Joint Authors
Imaizumi, Tsutomu
Kai, Hisashi
Ikeda, Ayami
Yasuoka, Suguru
Kage, Masayoshi
Kajimoto, Hidemi
Source
International Journal of Vascular Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
The heart consists of various kinds of cell components.
However, it has not been feasible to separately analyze the gene expression of individual components.
The laser microdissection (LMD) method, a new technology to collect target cells from the microscopic regions, has been used for malignancies.
We sought to establish a method to selectively collect the muscular and vascular regions from the heart sections and to compare the marker gene expressions with this method.
Methods and Results.
Frozen left ventricle sections were obtained from Wistar-Kyoto rats (WKY) and stroke-prone spontaneously hypertensive rats (SHR-SP) at 24 weeks of age.
Using the LMD method, the muscular and vascular regions were selectively collected under microscopic guidance.
Real-time RT-PCR analysis showed that brain-type natriuretic peptide (BNP), a marker of cardiac myocytes, was expressed in the muscular samples, but not in the vascular samples, whereas α-smooth muscle actin, a marker of smooth muscle cells, was detected only in the vascular samples.
Moreover, SHR-SP had significantly greater BNP upregulation than WKY (P<0.05) in the muscular samples.
Conclusions.
The LMD method enabled us to separately collect the muscular and vascular samples from myocardial sections and to selectively evaluate mRNA expressions of the individual tissue component.
American Psychological Association (APA)
Ikeda, Ayami& Kai, Hisashi& Kajimoto, Hidemi& Yasuoka, Suguru& Kage, Masayoshi& Imaizumi, Tsutomu. 2012. Selective Gene Expression Analysis of Muscular and Vascular Components in Hearts Using Laser Microdissection Method. International Journal of Vascular Medicine،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-504379
Modern Language Association (MLA)
Ikeda, Ayami…[et al.]. Selective Gene Expression Analysis of Muscular and Vascular Components in Hearts Using Laser Microdissection Method. International Journal of Vascular Medicine No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-504379
American Medical Association (AMA)
Ikeda, Ayami& Kai, Hisashi& Kajimoto, Hidemi& Yasuoka, Suguru& Kage, Masayoshi& Imaizumi, Tsutomu. Selective Gene Expression Analysis of Muscular and Vascular Components in Hearts Using Laser Microdissection Method. International Journal of Vascular Medicine. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-504379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504379