5-Lipoxygenase-Activating Protein as a Modulator of Olanzapine-Induced Lipid Accumulation in Adipocyte
Joint Authors
Manev, Hari
Dzitoyeva, Svetlana
Chen, Hu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Experiments were performed in 3T3-L1 preadipocytes differentiated in vitro into adipocytes.
Cells were treated with olanzapine and a 5-lipoxygenase (5-LOX) activating protein (FLAP) inhibitor MK-886.
Lipid content was measured using an Oil Red O assay; 5-LOX and FLAP mRNA content was measured using quantitative real-time PCR; the corresponding protein contents were measured using quantitative Western blot assay.
Olanzapine did not affect the cell content of 5-LOX mRNA and protein; it decreased FLAP mRNA and protein content at day five but not 24 hours after olanzapine addition.
In the absence of MK-886, low concentrations of olanzapine increased lipid content only slightly, whereas a 56% increase was induced by 50 μM olanzapine.
A 5-day cotreatment with 10 μM MK-886 potentiated the lipid increasing action of low concentrations of olanzapine.
In contrast, in the presence of 50 μM olanzapine nanomolar and low micromolar concentrations of MK-886 reduced lipid content.
These data suggest that FLAP system in adipocytes is affected by olanzapine and that it may modify how these cells respond to the second-generation antipsychotic drugs (SGADs).
Clinical studies could evaluate whether the FLAP/5-LOX system could play a role in setting a variable individual susceptibility to the metabolic side effects of SGADs.
American Psychological Association (APA)
Dzitoyeva, Svetlana& Chen, Hu& Manev, Hari. 2013. 5-Lipoxygenase-Activating Protein as a Modulator of Olanzapine-Induced Lipid Accumulation in Adipocyte. Journal of Lipids،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-504491
Modern Language Association (MLA)
Dzitoyeva, Svetlana…[et al.]. 5-Lipoxygenase-Activating Protein as a Modulator of Olanzapine-Induced Lipid Accumulation in Adipocyte. Journal of Lipids No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-504491
American Medical Association (AMA)
Dzitoyeva, Svetlana& Chen, Hu& Manev, Hari. 5-Lipoxygenase-Activating Protein as a Modulator of Olanzapine-Induced Lipid Accumulation in Adipocyte. Journal of Lipids. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-504491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504491