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Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes : Synergistic Roles of L-FABP and Glucose
Joint Authors
Landrock, Danilo
Martin, Gregory G.
McIntosh, Avery L.
Landrock, Kerstin K.
Petrescu, Anca D.
Schroeder, Friedhelm
Huang, Huan
Kier, Ann B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-26
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
While TOFA (acetyl CoA carboxylase inhibitor) and C75 (fatty acid synthase inhibitor) prevent lipid accumulation by inhibiting fatty acid synthesis, the mechanism of action is not simply accounted for by inhibition of the enzymes alone.
Liver fatty acid binding protein (L-FABP), a mediator of long chain fatty acid signaling to peroxisome proliferator-activated receptor-α (PPARα) in the nucleus, was found to bind TOFA and its activated CoA thioester, TOFyl-CoA, with high affinity while binding C75 and C75-CoA with lower affinity.
Binding of TOFA and C75-CoA significantly altered L-FABP secondary structure.
High (20 mM) but not physiological (6 mM) glucose conferred on both TOFA and C75 the ability to induce PPARα transcription of the fatty acid β-oxidative enzymes CPT1A, CPT2, and ACOX1 in cultured primary hepatocytes from wild-type (WT) mice.
However, L-FABP gene ablation abolished the effects of TOFA and C75 in the context of high glucose.
These effects were not associated with an increased cellular level of unesterified fatty acids but rather by increased intracellular glucose.
These findings suggested that L-FABP may function as an intracellular fatty acid synthesis inhibitor binding protein facilitating TOFA and C75-mediated induction of PPARα in the context of high glucose at levels similar to those in uncontrolled diabetes.
American Psychological Association (APA)
Huang, Huan& McIntosh, Avery L.& Martin, Gregory G.& Petrescu, Anca D.& Landrock, Kerstin K.& Landrock, Danilo…[et al.]. 2013. Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes : Synergistic Roles of L-FABP and Glucose. PPAR Research،Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-504584
Modern Language Association (MLA)
Huang, Huan…[et al.]. Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes : Synergistic Roles of L-FABP and Glucose. PPAR Research No. 2013 (2013), pp.1-22.
https://search.emarefa.net/detail/BIM-504584
American Medical Association (AMA)
Huang, Huan& McIntosh, Avery L.& Martin, Gregory G.& Petrescu, Anca D.& Landrock, Kerstin K.& Landrock, Danilo…[et al.]. Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes : Synergistic Roles of L-FABP and Glucose. PPAR Research. 2013. Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-504584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504584