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Directive Production of Pullulan by Altering Cheap Source of Carbons and Nitrogen at 5 L Bioreactor Level
Joint Authors
Tiwari, D. P.
Singh, Bhanu P.
Dubey, Kashyap Kumar
Sheoran, Sunil K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Engineering Sciences and Information Technology
Abstract EN
In the present paper, attempts have been made to control the production of pullulan by supplementing commercial source of carbons and protein, timely.
Pullulan production was regulated by supplying full fat soya flour and hydrolyzed soya extract, individually and in combination.
Pullulan quantification was assayed for sensitivity to pullulanase.
Aureobasidium pullulans was found to produce 125.7 gL−1 of pullulan.
The rotation speed of shake flask, the pH of broth, and the supply of air were maintained at 180 rpm, 5.9, and 1.5 vvm air, respectively.
The effect of carbons and lipids on pullulan production was noticed to be substrate specific.
However, after the lapse of 36 h, addition of full fat soya floor and hydrolyzed soya extract in combination enhanced the pullulan production 125.7 gL−1.
Besides this, pH of broth was also noticed as a critical factor in monitoring pullulan biosynthesis.
The newly isolated mutant Aureobasidium pullulans, having high potential for pullulan production as compared to existing data, can be well used for commercialization of pullulan.
American Psychological Association (APA)
Sheoran, Sunil K.& Dubey, Kashyap Kumar& Tiwari, D. P.& Singh, Bhanu P.. 2012. Directive Production of Pullulan by Altering Cheap Source of Carbons and Nitrogen at 5 L Bioreactor Level. ISRN Chemical Engineering،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-504643
Modern Language Association (MLA)
Sheoran, Sunil K.…[et al.]. Directive Production of Pullulan by Altering Cheap Source of Carbons and Nitrogen at 5 L Bioreactor Level. ISRN Chemical Engineering No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-504643
American Medical Association (AMA)
Sheoran, Sunil K.& Dubey, Kashyap Kumar& Tiwari, D. P.& Singh, Bhanu P.. Directive Production of Pullulan by Altering Cheap Source of Carbons and Nitrogen at 5 L Bioreactor Level. ISRN Chemical Engineering. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-504643
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504643