Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages
Joint Authors
Wu, Zhongdao
Hu, Shaomin
Yang, Linlin
Mak, Nai Ki
Fung, Ming Chui
Leung, Kwok Nam
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Sj16 is an anti-inflammatory protein identified from Schistosoma japonicum.
Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation.
In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated.
Our data demonstrated that rSj16 expressed and purified from Escherichia coli could suppress the proliferation of the WEHI-3B JCS cells in a time- and concentration-dependent manner, while not affect the viability of the cells.
Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases.
The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity.
Furthermore, our results revealed that rSj16 biased the colony formation of mouse bone marrow cells towards macrophage linage.
American Psychological Association (APA)
Hu, Shaomin& Yang, Linlin& Wu, Zhongdao& Mak, Nai Ki& Leung, Kwok Nam& Fung, Ming Chui. 2010. Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-504655
Modern Language Association (MLA)
Hu, Shaomin…[et al.]. Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-504655
American Medical Association (AMA)
Hu, Shaomin& Yang, Linlin& Wu, Zhongdao& Mak, Nai Ki& Leung, Kwok Nam& Fung, Ming Chui. Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-504655
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504655
