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Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication
Joint Authors
Bryant-Friedrich, Amanda C.
Amato, Nicholas J.
Mwai, Christopher N.
Mueser, Timothy C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Damaged DNA, generated by the abstraction of one of five hydrogen atoms from the 2′-deoxyribose ring of the nucleic acid, can contain a variety of lesions, some of which compromise physiological processes.
Recently, DNA damage, resulting from the formation of a C3′-thymidinyl radical in DNA oligomers, was found to be dependent on nucleic acid structure.
Architectures relevant to DNA replication were observed to generate larger amounts of strand-break and 1-(2′-deoxy-β-D-threo-pentofuranosyl)thymidine formation than that observed for duplex DNA.
To understand how this damage can affect the integrity of DNA, the impact of C3′-thymidinyl radical derived lesions on DNA stability and structure was characterized using biophysical methods.
DNA architectures evaluated include duplex DNA (dsDNA), single 3′ or 5′-overhangs (OvHgs), and forks.
Thermal melting analysis and differential scanning calorimetry measurements indicate that an individual 3′-OvHg is more destabilizing than a 5′-OvHg.
The presence of a terminal 3′ or 5′ phosphate decreases the ΔG25 to the same extent, while the effect of the phosphate at the ss-dsDNA junction of OvHgs is dependent on sequence.
Additionally, the effect of 1-(2′-deoxy-β-D-threo-pentofuranosyl)thymidine is found to depend on DNA architecture and proximity to the 3′ end of the damaged strand.
American Psychological Association (APA)
Amato, Nicholas J.& Mwai, Christopher N.& Mueser, Timothy C.& Bryant-Friedrich, Amanda C.. 2013. Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-504715
Modern Language Association (MLA)
Amato, Nicholas J.…[et al.]. Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication. Journal of Nucleic Acids No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-504715
American Medical Association (AMA)
Amato, Nicholas J.& Mwai, Christopher N.& Mueser, Timothy C.& Bryant-Friedrich, Amanda C.. Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-504715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504715