Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae)
Joint Authors
Venkatasubramanian, Padma
Balasubramani, Subramani Paranthaman
Devaiah, Kambiranda
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash.
Tubers of Ipomoea mauritiana Jacq.
(Convolvulaceae), Pueraria tuberosa (Roxb.
ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L.
(Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug.
DNA-based markers have been developed to distinguish I.
mauritiana from the other Vidari candidates.
A putative 600-bp polymorphic sequence, specific to I.
mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique.
Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon.
The SCAR primers produced a specific 323-bp amplicon in authentic I.
mauritiana and not in the allied species.
American Psychological Association (APA)
Devaiah, Kambiranda& Balasubramani, Subramani Paranthaman& Venkatasubramanian, Padma. 2011. Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae). Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-504777
Modern Language Association (MLA)
Devaiah, Kambiranda…[et al.]. Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae). Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-504777
American Medical Association (AMA)
Devaiah, Kambiranda& Balasubramani, Subramani Paranthaman& Venkatasubramanian, Padma. Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae). Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-504777
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504777
