Laccase Production from a Temperature and pH Tolerant Fungal Strain of Trametes hirsuta (MTCC 11397)
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Laccase production by a temperature and pH tolerant fungal strain (GBPI-CDF-03) isolated from a glacial site in Indian Himalayan Region (IHR) has been investigated.
The fungus developed white cottony mass on potato dextrose agar and revealed thread-like mycelium under microscope.
ITS region analysis of fungus showed its 100% similarity with Trametes hirsuta.
The fungus tolerated temperature from 4 to 48°C ± 2 (25°C opt.) and pH 3–13 (5–7 opt.).
Molecular weight of laccase was determined approximately 45 kDa by native PAGE.
Amplification of laccase gene fragment (corresponding to the copper-binding conserved domain) contained 200 bp.
The optimum pH for laccase production, at optimum growth temperature, was determined between 5.5 and 7.5.
In optimization experiments, fructose and ammonium sulfate were found to be the best carbon and nitrogen sources, respectively, for enhancing the laccase production.
Production of laccase was favored by high carbon/nitrogen ratio.
Addition of CuSO4 (up to 1.0 mM) induced laccase production up to 2-fold, in case of 0.4 mM concentration.
Addition of organic solvents also induced the production of laccase; acetone showed the highest (2-fold) induction.
The study has implications in bioprospecting of ecologically resilient microbial strains.
American Psychological Association (APA)
Dhakar, Kusum& Pandey, Anita. 2013. Laccase Production from a Temperature and pH Tolerant Fungal Strain of Trametes hirsuta (MTCC 11397). Enzyme Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-504794
Modern Language Association (MLA)
Dhakar, Kusum& Pandey, Anita. Laccase Production from a Temperature and pH Tolerant Fungal Strain of Trametes hirsuta (MTCC 11397). Enzyme Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-504794
American Medical Association (AMA)
Dhakar, Kusum& Pandey, Anita. Laccase Production from a Temperature and pH Tolerant Fungal Strain of Trametes hirsuta (MTCC 11397). Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-504794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504794