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RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells
Joint Authors
Sun, Yuncheng
Li, Tiejun
Jing, Jiaona
Li, Ping
Guan, Huaijin
Hu, Jianyan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Epithelial-msenchymal transition (EMT) contributes to posterior capsule opacification (PCO) type of cataract.
Transcription factors Snail is a key trigger of EMT activated by transforming growth factor β (TGFβ).
This study was done to investigate the effect of Snail targeting siRNA on TGFβ2-induced EMT in human lens epithelial cells.
TGFβ2 treatment of cultured human epithelial cell line (HLEB3) upregulated the expression of Snail and the EMT relevant molecules such as vimentin and α-SMA but downregulated the expression of keratin and E-cadherin.
After the stimulation of TGFβ2, the HLEB3 cells became fibroblast-like in morphology, and the junctions of cell-cell disappeared.
TGFβ2 treatment also enhanced migration ability of HLEB3 cells.
TGFβ2-induced Snail expression and EMT were significantly inhibited by Snail siRNA.
By analyzing the response characteristics of HLEB3 in TGFβ2-induced EMT model with/without Snail-specific siRNA, we concluded that Snail is an element in the EMT of HLEB3 cells induced by TGFβ2.
Snail siRNA targeting can block the induced EMT and therefore has the potential to suppress the development of PCO.
American Psychological Association (APA)
Li, Ping& Jing, Jiaona& Hu, Jianyan& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. 2013. RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells. Journal of Ophthalmology،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-504800
Modern Language Association (MLA)
Li, Ping…[et al.]. RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells. Journal of Ophthalmology No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-504800
American Medical Association (AMA)
Li, Ping& Jing, Jiaona& Hu, Jianyan& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells. Journal of Ophthalmology. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-504800
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504800