Fabrication of Biosensor Based on PolyanilineGold Nanorod Composite
Joint Authors
Tamer, Uğur
Torul, Hilal
Temur, Erhan
Seçkin, Ali İhsan
Source
International Journal of Electrochemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This present paper describes a new approach to fabricate a new amperometric sensor for the determination of glucose.
Polyaniline (PANI) film doped with colloidal gold nanorod particles has been used to immobilize glucose oxidase by glutaraldehyde.
The polyaniline/gold nanorod composite structure gave an excellent matrix for enzyme immobilization due to the large specific surface area and higher electroactivity.
The composite has been characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), and surface-enhanced Raman spectroscopy (SERS).
The SERS spectrum of the surface-immobilized glucose oxidase and the spectrum of the native enzyme indicate that the main feature of the native structure of glucose oxidase was conserved after being immobilized on the polymer matrix.
The amperometric response was measured as a function of concentration of glucose at a potential of 0.6 V versus Ag/AgCl in 0.1 M phosphate buffer at pH 6.4.
Linear range of the calibration curve was from 17.6 μM to 1 mM with a sensitivity of 13.8 μA⋅mM−1⋅cm−2 and a limit of detection (LOD) of 5.8 μM.
The apparent Michaelis-Menten constant KM was calculated as 1.0 mM and the response time was less than 3 seconds.
American Psychological Association (APA)
Tamer, Uğur& Seçkin, Ali İhsan& Temur, Erhan& Torul, Hilal. 2011. Fabrication of Biosensor Based on PolyanilineGold Nanorod Composite. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-504869
Modern Language Association (MLA)
Tamer, Uğur…[et al.]. Fabrication of Biosensor Based on PolyanilineGold Nanorod Composite. International Journal of Electrochemistry No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-504869
American Medical Association (AMA)
Tamer, Uğur& Seçkin, Ali İhsan& Temur, Erhan& Torul, Hilal. Fabrication of Biosensor Based on PolyanilineGold Nanorod Composite. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-504869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504869