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Specificity of Methylation Assays in Cancer Research : A Guideline for Designing Primers and Probes
Joint Authors
Radpour, Ramin
Zhong, Xiao Yan
Kohler, Corina
Barekati, Zeinab
Source
Obstetrics and Gynecology International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers.
A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer).
However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test.
This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling.
The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step.
American Psychological Association (APA)
Barekati, Zeinab& Radpour, Ramin& Kohler, Corina& Zhong, Xiao Yan. 2010. Specificity of Methylation Assays in Cancer Research : A Guideline for Designing Primers and Probes. Obstetrics and Gynecology International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-504954
Modern Language Association (MLA)
Barekati, Zeinab…[et al.]. Specificity of Methylation Assays in Cancer Research : A Guideline for Designing Primers and Probes. Obstetrics and Gynecology International No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-504954
American Medical Association (AMA)
Barekati, Zeinab& Radpour, Ramin& Kohler, Corina& Zhong, Xiao Yan. Specificity of Methylation Assays in Cancer Research : A Guideline for Designing Primers and Probes. Obstetrics and Gynecology International. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-504954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504954