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Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta
Joint Authors
Sherrer, Shanen M.
Burgers, Peter M. J.
Zhang, Likui
Taylor, John-Stephen
Suo, Zucai
Brown, Jessica A.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Understanding polymerase fidelity is an important objective towards ascertaining the overall stability of an organism's genome.
Saccharomyces cerevisiae DNA polymerase η (yPolη), a Y-family DNA polymerase, is known to efficiently bypass DNA lesions (e.g., pyrimidine dimers) in vivo.
Using pre-steady-state kinetic methods, we examined both full-length and a truncated version of yPolη which contains only the polymerase domain.
In the absence of yPolη's C-terminal residues 514–632, the DNA binding affinity was weakened by 2-fold and the base substitution fidelity dropped by 3-fold.
Thus, the C-terminus of yPolη may interact with DNA and slightly alter the conformation of the polymerase domain during catalysis.
In general, yPolη discriminated between a correct and incorrect nucleotide more during the incorporation step (50-fold on average) than the ground-state binding step (18-fold on average).
Blunt-end additions of dATP or pyrene nucleotide 5′-triphosphate revealed the importance of base stacking during the binding of incorrect incoming nucleotides.
American Psychological Association (APA)
Brown, Jessica A.& Zhang, Likui& Sherrer, Shanen M.& Taylor, John-Stephen& Burgers, Peter M. J.& Suo, Zucai. 2010. Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-505063
Modern Language Association (MLA)
Brown, Jessica A.…[et al.]. Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta. Journal of Nucleic Acids No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-505063
American Medical Association (AMA)
Brown, Jessica A.& Zhang, Likui& Sherrer, Shanen M.& Taylor, John-Stephen& Burgers, Peter M. J.& Suo, Zucai. Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-505063
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505063