Morphological Analysis and Interaction of Chlorophyll and BSA
Joint Authors
Silva, Josmary R.
Trescher, Tarquin F.
da Silva, Romário J.
Gorza, Filipe D. S.
Pedro, Graciela C.
de Souza, Nara C.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Interactions between proteins and drugs, which can lead to formation of stable drug-protein complexes, have important implications on several processes related to human health.
These interactions can affect, for instance, free concentration, biological activity, and metabolism of the drugs in the blood stream.
Here, we report on the UV-Visible spectroscopic investigation on the interaction of bovine serum albumin (BSA) with chlorophyll (Chl) in aqueous solution under physiological conditions.
Binding constants at different temperatures—obtained by using the Benesi-Hildebrand equation—were found to be of the same order of magnitude (~104 M−1) indicating low affinity of Chl with BSA.
We have found a hyperchromism, which suggested an interaction between BSA and Chl occurring through conformational changes of BSA caused by exposition of tryptophan to solvent.
Films from BSA and Chl obtained at different Chl concentrations showed fractal structures, which were characterized by fractal dimension calculated from microscopic image analysis.
American Psychological Association (APA)
Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.& da Silva, Romário J.& Silva, Josmary R.& de Souza, Nara C.. 2014. Morphological Analysis and Interaction of Chlorophyll and BSA. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-505137
Modern Language Association (MLA)
Gorza, Filipe D. S.…[et al.]. Morphological Analysis and Interaction of Chlorophyll and BSA. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-505137
American Medical Association (AMA)
Gorza, Filipe D. S.& Pedro, Graciela C.& Trescher, Tarquin F.& da Silva, Romário J.& Silva, Josmary R.& de Souza, Nara C.. Morphological Analysis and Interaction of Chlorophyll and BSA. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-505137
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505137