Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form
Joint Authors
Jadhav, Monika L.
Tidme, Shripad K.
Girase, Manoj V.
Junagade, Manish S.
Source
International Journal of Spectroscopy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form.
The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, λmax for valsartan and hydrochlorothiazide, respectively.
The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm (λmax of hydrochlorothiazide).
The methods were found to be linear between the range of 5–30 µg/mL for valsartan and 4–24 μg/mL for hydrochlorothiazide using 0.1 N NaOH as solvent.
The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions.
The precision (intraday, interday) of methods was found within limits (RSD<2%).
It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.
American Psychological Association (APA)
Jadhav, Monika L.& Girase, Manoj V.& Tidme, Shripad K.& Junagade, Manish S.. 2014. Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form. International Journal of Spectroscopy،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-505232
Modern Language Association (MLA)
Jadhav, Monika L.…[et al.]. Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form. International Journal of Spectroscopy No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-505232
American Medical Association (AMA)
Jadhav, Monika L.& Girase, Manoj V.& Tidme, Shripad K.& Junagade, Manish S.. Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form. International Journal of Spectroscopy. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-505232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505232