Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System
Joint Authors
Davoudi, Noushin
Mirzaahmadi, Sina
Kazemi, Bahram
Mirzahoseini, Hasan
Bandehpour, Mojgan
Asaadi-Tehrani, Golnaz
Hosseinzadeh, Nahid
Tahmasbi, Leila
Parivar, Kazim
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression.
In the current study, the authors used a novel protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host for expression of FVII.
Plasmid containing cDNA encoding full-length human FVII was introduced into Lizard Leishmania and positive transfectants were analyzed by SDS-PAGE and Western blot analysis.
Furthermore, biological activity of purified protein was detected by PT assay.
The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level.
Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII.
Western blot analysis was carried out.
Clotting time in PT assay was reduced about 30 seconds with the purified rFVII.
In Conclusion, this study has demonstrated, for the first time, that Leishmania cells can be used as an expression system for producing recombinant FVII.
American Psychological Association (APA)
Mirzaahmadi, Sina& Asaadi-Tehrani, Golnaz& Bandehpour, Mojgan& Davoudi, Noushin& Tahmasbi, Leila& Hosseinzadeh, Nahid…[et al.]. 2011. Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-505235
Modern Language Association (MLA)
Mirzaahmadi, Sina…[et al.]. Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-505235
American Medical Association (AMA)
Mirzaahmadi, Sina& Asaadi-Tehrani, Golnaz& Bandehpour, Mojgan& Davoudi, Noushin& Tahmasbi, Leila& Hosseinzadeh, Nahid…[et al.]. Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-505235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505235