Optimization and Immobilization of Purified Labeo rohita Visceral Protease by Entrapment Method
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The purified fish visceral protease enzyme was immobilized by using various concentrations of sodium alginate and calcium chloride to optimize the best concentration for the formation of the beads.
Then it was characterized by assaying the optimal pH, temperature, storage stability and reusability.
The results on immobilization with sodium alginate and calcium chloride showed that a combination of 2% sodium alginate and 0.3 M calcium chloride weas found to be the optimum concentration for the formation of spherical and stable beads, this gave a maximal entrapped activity of 48.31%, and there was no change in the optimum pH 8.0 and temperature 40°C of protease before and after entrapment.
The results on stability and reusability indicated that it was stable at 4°C retaining 100% residual activity after 5 days of storage and 67% loss of activity after ten days of storage and it retained 100% residual activity on the first reuse, 75% residual activity on the second reuse, 25% residual activity on the third use and complete loss in the activity on the fourth reuse.
American Psychological Association (APA)
Geethanjali, S.& Subash, Anitha. 2013. Optimization and Immobilization of Purified Labeo rohita Visceral Protease by Entrapment Method. Enzyme Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-505252
Modern Language Association (MLA)
Geethanjali, S.& Subash, Anitha. Optimization and Immobilization of Purified Labeo rohita Visceral Protease by Entrapment Method. Enzyme Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-505252
American Medical Association (AMA)
Geethanjali, S.& Subash, Anitha. Optimization and Immobilization of Purified Labeo rohita Visceral Protease by Entrapment Method. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-505252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505252