Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1
Joint Authors
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Information Technology and Computer Science
Abstract EN
The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites.
Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose.
GI is used widely for the production of High-Fructose Corn Syrup (HFCS).
HFCS is used as a sweetener in food and pharmaceutical industries.
Streptomyces are well-known producers of numerous enzymes including glucose isomerase.
An array of 75 isolates was screened for the production of glucose isomerase.
The isolate Streptomyces sp.
SB-P1 was found to produce maximum amount of extracellular GI.
Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers.
Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production.
Quantitative effect of carbon, nitrogen, and inducer on GI was also determined.
Plackett-Burman design was used to study the effect of different medium ingredients.
Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.
American Psychological Association (APA)
Bhasin, Sheetal& Modi, H. A.. 2012. Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505265
Modern Language Association (MLA)
Bhasin, Sheetal& Modi, H. A.. Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1. Biotechnology Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-505265
American Medical Association (AMA)
Bhasin, Sheetal& Modi, H. A.. Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505265