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Genetic Heterogeneity of Breast Cancer Metastasis May Be Related to miR-21 Regulation of TIMP-3 in Translation
Joint Authors
Li, Jianyi
Li, Dan
Jia, Shi
Kang, Ye
Ma, Yan
Zhang, Wenhai
Tian, Rui
Zhang, Yang
Source
International Journal of Surgical Oncology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
MicroRNAs are noncoding RNA molecules that posttranscriptionally regulated expression of target gene and implicate the progress of cancer proliferation, differentiation, and apoptosis.
The aim of this study is to determine whether microRNA-21 (miR-21), a specific microRNA implicated in multiple aspects of carcinogenesis, promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 (TIMP-3) gene.
Methods.
miR-21 of serum and tissue from 40 patients (30 patients with breast cancer) were detected by real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR).
TIMP-3 of tissue from the patient was tested by real-time RT-qPCR.
Protein expression of TIMP-3 was evaluated by western blotting.
Correlation analysis was performed between miR-21 and TIMP-3.
Results.
Of the 40 samples from tissue and serum analyzed, the miR-21 expression was significantly higher in high invasion metastasis group (HIMG) that in low invasion metastasis group (LIMG); the latter was higher than that in normal group (NG).
Additionally, the TIMP-3 expression was significantly lower in HIMG than in LIMG; the latter was lower than that in NG.
There was significantly inverse correlation between miR-21 and TIMP-3 extracted from tissue.
Conclusion.
Our data suggest that miR-21 could promote metastasis in breast cancer via the regulation of TIMP3 translation, and there was consistency between miR-21 of serum and miR-21 in tissue.
American Psychological Association (APA)
Li, Jianyi& Zhang, Yang& Zhang, Wenhai& Jia, Shi& Tian, Rui& Kang, Ye…[et al.]. 2013. Genetic Heterogeneity of Breast Cancer Metastasis May Be Related to miR-21 Regulation of TIMP-3 in Translation. International Journal of Surgical Oncology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-505349
Modern Language Association (MLA)
Li, Jianyi…[et al.]. Genetic Heterogeneity of Breast Cancer Metastasis May Be Related to miR-21 Regulation of TIMP-3 in Translation. International Journal of Surgical Oncology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-505349
American Medical Association (AMA)
Li, Jianyi& Zhang, Yang& Zhang, Wenhai& Jia, Shi& Tian, Rui& Kang, Ye…[et al.]. Genetic Heterogeneity of Breast Cancer Metastasis May Be Related to miR-21 Regulation of TIMP-3 in Translation. International Journal of Surgical Oncology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-505349
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505349