Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels
Joint Authors
Armour, C. L.
Tong, K.
Moir, Lyn M.
Alkhouri, H.
Cha, V.
Hughes, J. M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In asthma, the airway smooth muscle (ASM) produces CXCL10 which may attract CXCR3+ mast/T cells to it.
Our aim was to investigate the effects of mast cell products on ASM cell CXCL10 production.
ASM cells from people with and without asthma were stimulated with IL-1β, TNF-α, and/or IFNγ and treated with histamine (1–100 μM) ± chlorpheniramine (H1R antagonist; 1 μM) or ranitidine (H2R antagonist; 50 μM) or tryptase (1 nM) ± leupeptin (serine protease inhibitor; 50 μM), heat-inactivated tryptase, or vehicle for 4 h or 24 h.
Human lung mast cells (MC) were isolated and activated with IgE/anti-IgE and supernatants were collected after 2 h or 24 h.
The supernatants were added to ASM cells for 48 h and ASM cell CXCL10 production detected using ELISA (protein) and real-time PCR (mRNA).
Histamine reduced IL-1β/TNF-α-induced CXCL10 protein, but not mRNA, levels independent of H1 and H2 receptor activation, whereas tryptase and MC 2 h supernatants reduced all cytokine-induced CXCL10.
Tryptase also reduced CXCL10 levels in a cell-free system.
Leupeptin inhibited the effects of tryptase and MC 2 h supernatants.
MC 24 h supernatants contained TNF-α and amplified IFNγ-induced ASM cell CXCL10 production.
This is the first evidence that MC can regulate ASM cell CXCL10 production and its degradation.
Thus MC may regulate airway myositis in asthma.
American Psychological Association (APA)
Alkhouri, H.& Cha, V.& Tong, K.& Moir, Lyn M.& Armour, C. L.& Hughes, J. M.. 2014. Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels. Journal of Allergy،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-505353
Modern Language Association (MLA)
Alkhouri, H.…[et al.]. Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels. Journal of Allergy No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-505353
American Medical Association (AMA)
Alkhouri, H.& Cha, V.& Tong, K.& Moir, Lyn M.& Armour, C. L.& Hughes, J. M.. Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels. Journal of Allergy. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-505353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505353