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Development and Application of an ELISA Assay Using ExcretionSecretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease
Joint Authors
Ward, Brian J.
Ndao, Momar
Jorquera, Alicia
Rodríguez, Jessicca
Berrizbeitia, Mariolga
Figueroa, Milagros
Romero, Leomerys
Figuera, Maria A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants.
A panel of 120 serum samples from subjects with confirmed Chagas disease (n=50), healthy controls (n=50), and patients with other parasitic diseases (n=20) was used to evaluate the new ESEA-based ELISA (ELISAESEA).
This new test had excellent sensitivity (98%) and acceptable specificity (88%).
Cross-reactivity was observed largely in sera from subjects with Leishmania and Ascaris infections.
Using Western blotting and epimastigotes from two distinct T.
cruzi isolates, several polypeptide bands with molecular masses ranging from 50 to 220 kDa were detected in pooled chagasic sera.
However, the band pattern for each isolate was different.
These data suggest that an inexpensive and technically simple ELISA based on ESEA antigens is a promising new tool for the diagnosis of Chagas disease.
American Psychological Association (APA)
Berrizbeitia, Mariolga& Figueroa, Milagros& Ward, Brian J.& Rodríguez, Jessicca& Jorquera, Alicia& Figuera, Maria A.…[et al.]. 2012. Development and Application of an ELISA Assay Using ExcretionSecretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease. Journal of Tropical Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505430
Modern Language Association (MLA)
Berrizbeitia, Mariolga…[et al.]. Development and Application of an ELISA Assay Using ExcretionSecretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease. Journal of Tropical Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-505430
American Medical Association (AMA)
Berrizbeitia, Mariolga& Figueroa, Milagros& Ward, Brian J.& Rodríguez, Jessicca& Jorquera, Alicia& Figuera, Maria A.…[et al.]. Development and Application of an ELISA Assay Using ExcretionSecretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease. Journal of Tropical Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505430
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505430