E. coli Endotoxin Modulates the Expression of Sirtuin Proteins in PBMC in Humans
Joint Authors
Leisser, Asha
Jew, Michael
Wolzt, Michael
Seper, Martin
Führlinger, Gerhard
Storka, Angela
Wang, Lisa
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Sirtuin (SIRT) proteins are class I histone deacetylases displaying gene regulatory functions in inflammatory, cancer, and metabolic diseases.
These SIRT actions involve the nuclear factor κB and its inhibitor IκB pathway.
However, the regulation of SIRT in vivo is still unclear.
Material and Methods.
In a human endotoxemia model, 20 healthy male subjects received an intravenous bolus of 2 ng/kg body weight Escherichia coli endotoxin (LPS).
SIRT expression was investigated in peripheral blood mononuclear cells (PBMC) with qPCR and Western blot before and 3 hours, 6 hours, and 24 hours after LPS challenge.
Additionally, SIRT regulation was studied in vitro in cultivated PBMC after incubation with 20 ng/mL LPS.
Results.
A downregulation by >40% of SIRT1 mRNA was detectable 3 hours after LPS and of SIRT3 mRNA 6 hours after LPS.
SIRT3, IκBα, and IκB-β protein expressions were decreased 3 and 6 hours after LPS.
SIRT2 mRNA or protein expression did not change following LPS.
These findings were consistent in vitro and associated with augmented phosphorylation of IκB-β.
Discussion.
In this E.
coli endotoxemia model, SIRT1 and SIRT3 mRNA expressions in PBMC in humans were reduced after LPS challenge.
This suggests that SIRT may represent an inflammatory target protein in vivo.
American Psychological Association (APA)
Storka, Angela& Führlinger, Gerhard& Seper, Martin& Wang, Lisa& Jew, Michael& Leisser, Asha…[et al.]. 2013. E. coli Endotoxin Modulates the Expression of Sirtuin Proteins in PBMC in Humans. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-505530
Modern Language Association (MLA)
Storka, Angela…[et al.]. E. coli Endotoxin Modulates the Expression of Sirtuin Proteins in PBMC in Humans. Mediators of Inflammation No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-505530
American Medical Association (AMA)
Storka, Angela& Führlinger, Gerhard& Seper, Martin& Wang, Lisa& Jew, Michael& Leisser, Asha…[et al.]. E. coli Endotoxin Modulates the Expression of Sirtuin Proteins in PBMC in Humans. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-505530
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505530