The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain
Joint Authors
Vassaux, Georges
Hoffman, Chantal
Geist, Michel
Tran, Lucile
Rooke, Ronald
Conchon, Sophie
Gauttier, Vanessa
Judor, Jean-Paul
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA) vector to induce an immune response against a well-tolerated self-antigen.
Methods.
rMVA vectors expressing different form of α-fetoprotein (AFP) were produced and characterized.
Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membrane-bound, or an intracellular form.
The immune response was monitored by an IFNΓ ELISpot assay and antibody detection.
Results.
Vaccination with the membrane-associated form of AFP induced a stronger CD8+ T-cell response compared to the ones obtained with the MVA encoding the secreted or the intracellular forms of AFP.
Moreover, the vaccination with the membrane-bound AFP elicited the production of AFP-specific antibodies.
Conclusions.
The AFP transmembrane form is more immunogenic.
Expressing a membrane-bound form in the context of an MVA vaccination could enhance the immunogenicity of a self-antigen.
American Psychological Association (APA)
Tran, Lucile& Judor, Jean-Paul& Gauttier, Vanessa& Geist, Michel& Hoffman, Chantal& Rooke, Ronald…[et al.]. 2012. The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505587
Modern Language Association (MLA)
Tran, Lucile…[et al.]. The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-505587
American Medical Association (AMA)
Tran, Lucile& Judor, Jean-Paul& Gauttier, Vanessa& Geist, Michel& Hoffman, Chantal& Rooke, Ronald…[et al.]. The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505587