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Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro
Joint Authors
Polan, Mohammad Ali Akbor
Tang, Jia
Saito, Takashi
Hayashi, Keijiro
Izumikawa, Masanobu
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The aim of this study was to clarify the function of amelogenin, the major protein of enamel matrix derivative, on the proliferation, differentiation, and mineralization of cultured rat bone marrow stem cells (BMSCs), toward the establishment of future bone regenerative therapies.
No differences in the morphology of BMSCs or in cell numbers were found between amelogenin addition and additive-free groups.
The promotion of ALPase activity and the formation of mineralized nodules were detected at an early stage in amelogenin addition group.
In quantitative real-time RT-PCR, mRNA expression of osteopontin, osteonectin, and type I collagen was promoted for 0.5 hours and 24 hours by addition of amelogenin.
The mRNA expression of osteocalcin and DMP-1 was also stimulated for 24 hours and 0.5 hours, respectively, in amelogenin addition group.
These findings clearly indicate that amelogenin promoted the differentiation and mineralization of rat BMSCs but did not affect cell proliferation or cell morphology.
American Psychological Association (APA)
Izumikawa, Masanobu& Hayashi, Keijiro& Polan, Mohammad Ali Akbor& Tang, Jia& Saito, Takashi. 2012. Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-505674
Modern Language Association (MLA)
Izumikawa, Masanobu…[et al.]. Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-505674
American Medical Association (AMA)
Izumikawa, Masanobu& Hayashi, Keijiro& Polan, Mohammad Ali Akbor& Tang, Jia& Saito, Takashi. Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-505674
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505674