Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein
Joint Authors
Marchat, Laurence A.
López-Reyes, Israel
García-Rivera, Guillermina
Orozco, Esther
Bañuelos, Cecilia
Vincent, Olivier
Herranz, Silvia
López-Camarillo, César
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-17
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Eukaryotic endocytosis involves multivesicular bodies formation, which is driven by endosomal sorting complexes required for transport (ESCRT).
Here, we showed the presence and expression of homologous ESCRT genes in Entamoeba histolytica.
We cloned and expressed the Ehvps4 gene, an ESCRT member, to obtain the recombinant EhVps4 and generate specific antibodies, which immunodetected EhVps4 in cytoplasm of trophozoites.
Bioinformatics and biochemical studies evidenced that rEhVps4 is an ATPase, whose activity depends on the conserved E211 residue.
Next, we generated trophozoites overexpressing EhVps4 and mutant EhVps4-E211Q FLAG-tagged proteins.
The EhVps4-FLAG was located in cytosol and at plasma membrane, whereas the EhVps4-E211Q-FLAG was detected as abundant cytoplasmic dots in trophozoites.
Erythrophagocytosis, cytopathic activity, and hepatic damage in hamsters were not improved in trophozoites overexpressing EhVps4-FLAG.
In contrast, EhVps4-E211Q-FLAG protein overexpression impaired these properties.
The localization of EhVps4-FLAG around ingested erythrocytes, together with our previous results, strengthens the role for EhVps4 in E.
histolytica phagocytosis and virulence.
American Psychological Association (APA)
López-Reyes, Israel& García-Rivera, Guillermina& Bañuelos, Cecilia& Herranz, Silvia& Vincent, Olivier& López-Camarillo, César…[et al.]. 2010. Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-505751
Modern Language Association (MLA)
López-Reyes, Israel…[et al.]. Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-15.
https://search.emarefa.net/detail/BIM-505751
American Medical Association (AMA)
López-Reyes, Israel& García-Rivera, Guillermina& Bañuelos, Cecilia& Herranz, Silvia& Vincent, Olivier& López-Camarillo, César…[et al.]. Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-505751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505751