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Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells : Role of ROS Mediated DNA Damage and Apoptosis
Joint Authors
Pandey, Alok K.
Mittal, Sandeep
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-01
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Cerium oxide nanoparticles (CeO2 NPs) have promising industrial and biomedical applications.
In spite of their applications, the toxicity of these NPs in biological/physiological environment is a major concern.
Present study aimed to understand the molecular mechanism underlying the toxicity of CeO2 NPs on lung adenocarcinoma (A549) cells.
After internalization, CeO2 NPs caused significant cytotoxicity and morphological changes in A549 cells.
Further, the cell death was found to be apoptotic as shown by loss in mitochondrial membrane potential and increase in annexin-V positive cells and confirmed by immunoblot analysis of BAX, BCl-2, Cyt C, AIF, caspase-3, and caspase-9.
A significant increase in oxidative DNA damage was found which was confirmed by phosphorylation of p53 gene and presence of cleaved poly ADP ribose polymerase (PARP).
This damage could be attributed to increased production of reactive oxygen species (ROS) with concomitant decrease in antioxidant “glutathione (GSH)” level.
DNA damage and cell death were attenuated by the application of ROS and apoptosis inhibitors N-acetyl-L- cysteine (NAC) and Z-DEVD-fmk, respectively.
Our study concludes that ROS mediated DNA damage and cell cycle arrest play a major role in CeO2 NPs induced apoptotic cell death in A549 cells.
Apart from beneficial applications, these NPs also impart potential harmful effects which should be properly evaluated prior to their use.
American Psychological Association (APA)
Mittal, Sandeep& Pandey, Alok K.. 2014. Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells : Role of ROS Mediated DNA Damage and Apoptosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-505888
Modern Language Association (MLA)
Mittal, Sandeep& Pandey, Alok K.. Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells : Role of ROS Mediated DNA Damage and Apoptosis. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-505888
American Medical Association (AMA)
Mittal, Sandeep& Pandey, Alok K.. Cerium Oxide Nanoparticles Induced Toxicity in Human Lung Cells : Role of ROS Mediated DNA Damage and Apoptosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-505888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505888