A Novel and Sensitive Method for the Determination of Vitamin B2 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction
Joint Authors
Delpisheh, Ali
Shiri, Sabah
Abbasi, Alimohammad
Sajjadifar, Sami
Avazpour, Moayed
Khezeli, Tahere
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-08
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A novel, simple, and more sensitive spectrophotometric procedure has been developed for the determination of vitamin B2 (riboflavin) by an aqueous two-phase extraction (ATPE).
An ATPE is formed mostly by water and does not require an organic solvent.
Other ATPE components used in this study were the polymer, polyethylene glycol (PEG), and some salts such as Na2SO4 and Na2CO3.
The method is based on the interaction between vitamin B2 (riboflavin) and sodium sulfate (Na2SO4) in an acidic medium (pH 6.4).
The influences of effective parameters such as salt (type and concentration), polyethylene glycol (molecular weight and concentration), temperature, centrifuging time, and pH of the sample solution were studied and optimized.
The linear range was 1.3–320 ng/mL (R2=0.9991; n=10) with the relative standard deviation (RSD) for 60 ng/mL 3.68%.
The limit of detection (LOD) calculated from three times of standard deviation of blank were 0.2 ng/mL and recoveries from analysis of real samples between 94.82% and 103.98% were obtained for the determination of vitamin B2 (riboflavin) in urine and pharmaceutical samples.
American Psychological Association (APA)
Shiri, Sabah& Khezeli, Tahere& Sajjadifar, Sami& Delpisheh, Ali& Avazpour, Moayed& Abbasi, Alimohammad. 2013. A Novel and Sensitive Method for the Determination of Vitamin B2 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-505918
Modern Language Association (MLA)
Shiri, Sabah…[et al.]. A Novel and Sensitive Method for the Determination of Vitamin B2 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction. Journal of Chemistry No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-505918
American Medical Association (AMA)
Shiri, Sabah& Khezeli, Tahere& Sajjadifar, Sami& Delpisheh, Ali& Avazpour, Moayed& Abbasi, Alimohammad. A Novel and Sensitive Method for the Determination of Vitamin B2 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-505918
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505918