Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form
Joint Authors
Jain, P. S.
Bari, Sanjay B.
Bakhshi, A. R.
Surana, Sanjay J.
Source
Chromatography Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple, economic, selective, precise, and stability-indicating high-performance thin-layer chromatographic method for analysis of tamsulosin hydrochloride, both as a bulk drug and in formulations, was developed and validated according to ICH guidelines.
The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase while the solvent system consisted of toluene : methanol : triethylamine (3.5 : 1.2 : 0.2 v/v).
The system was found to give compact spot for drug (Rf value of 0.52 ± 0.02).
Densitometric analysis of tamsulosin was carried out in the absorbance mode at 280 nm.
The linear regression analysis data for the calibration plots showed good linear relationship, r2=0.9982±0.0012, with respect to peak area in the concentration range 400–2400 ng per spot.
The mean value ± SD of slope and intercept were 2.6553 ± 0.0173 and 777.7 ± 74.8 with respect to peak area.
The method was validated for precision, recovery, and robustness.
The limits of detection and quantitation were 20.49 and 62.10 ng per spot, respectively.
Tamsulosin was subjected to hydrolysis, oxidation, and thermal degradation which indicate the drug is susceptible to hydrolysis, oxidation, and heat.
Statistical analysis proves that the method is repeatable, selective, and accurate for the estimation of tamsulosin.
American Psychological Association (APA)
Bari, Sanjay B.& Bakhshi, A. R.& Jain, P. S.& Surana, Sanjay J.. 2011. Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form. Chromatography Research International،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-506009
Modern Language Association (MLA)
Bari, Sanjay B.…[et al.]. Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form. Chromatography Research International No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-506009
American Medical Association (AMA)
Bari, Sanjay B.& Bakhshi, A. R.& Jain, P. S.& Surana, Sanjay J.. Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form. Chromatography Research International. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-506009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506009