Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae
Joint Authors
Smedsgaard, Jørn
Nielsen, Jens
Hansen, Michael Adsetts Edberg
Chumnanpuen, Pramote
Source
International Journal of Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Metabolic footprinting offers a relatively easy approach to exploit the potentials of metabolomics for phenotypic characterization of microbial cells.
To capture the highly dynamic nature of metabolites, we propose the use of dynamic metabolic footprinting instead of the traditional method which relies on analysis at a single time point.
Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S.
cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates.
In order to analyze the dynamic mass spectrometry data, we developed the novel analysis methods that allow us to perform correlation analysis to identify metabolites that significantly correlate over time during growth on the different carbon sources.
Both positive and negative electrospray ionization (ESI) modes were performed to obtain the complete information about the metabolite content.
Using sparse principal component analysis (Sparse PCA), we further identified those pairs of metabolites that significantly contribute to the separation.
From the list of significant metabolite pairs, we reconstructed an interaction map that provides information of how different metabolic pathways have correlated patterns during growth on the different carbon sources.
American Psychological Association (APA)
Chumnanpuen, Pramote& Hansen, Michael Adsetts Edberg& Smedsgaard, Jørn& Nielsen, Jens. 2014. Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-506099
Modern Language Association (MLA)
Chumnanpuen, Pramote…[et al.]. Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae. International Journal of Genomics No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-506099
American Medical Association (AMA)
Chumnanpuen, Pramote& Hansen, Michael Adsetts Edberg& Smedsgaard, Jørn& Nielsen, Jens. Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-506099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506099